Establishment of serological test to detect antibody against ferret coronavirus

Establishment of serological test to detect antibody against ferret coronavirus

Since there is no available serological methods to detect antibodies to ferret coronavirus (FRCoV), an enzyme-linked immunosorbent assay (ELISA) using recombinant partial nucleocapsid (N) proteins of the ferret coronavirus (FRCoV) Yamaguchi-1 strain was developed to establish a serological method for detection of FRCoV infection. Many serum samples collected from ferrets recognized both a.a. 1-...

Development of a virus neutralisation test to detect antibodies against Schmallenberg virus and serological results in suspect and infected herds

BACKGROUND At the end of 2011, a new orthobunyavirus, tentatively named Schmallenberg virus (SBV), was discovered in Germany. This virus has since been associated with clinical signs of decreased milk production, watery diarrhoea and fever in dairy cows, and subsequently also with congenital malformations in calves, lambs and goat kids. In affected countries, initial surveillance for the infect...

Fluorescent antibody test for the serological diagnosis of gonorrhea.

An indirect fluorescent antibody technique has been developed for the serological diagnosis of gonorrhea. The selected strain(s) of Neisseria gonorrhoeae possesses a heat-labile surface antigen (L-antigen). Sera are diluted 1:10, and an aliquot is hear inactivated at 59 C for 30 min. The treated and untreated aliquots are then examined for human immunoglobulin G anti-L-antigen. In a prelimiary ...

Serological survey of sheep in Iran for Type specific Antibody to Bluetongue virus

A Serological Comparison of Bovine Coronavirus Isolates

viii Chapter 1: Introduction 1 Chapter 2: Materials and Methods 63 Chapter 3: Isolation and Growth of Bovine 113 Coronaviruses Chapter 4: Monoclonal Antibodies to Bovine 133 Coronavirus Chapter 5: Structural Proteins of Bovine 172 Coronavirus Chapter 6: Strain Comparison of Bovine Coronaviruses 212 Chapter 7: Animal Models of Bovine Coronavirus 259 Infection Chapter 8: General Discussion 285 Ap...